June 2015 - New Publication
Live imaging and modeling of inner nuclear membrane targeting reveals its molecular requirements in mammalian cells.
Andrea Boni and coworkers used a new reporter for real-time imaging of membrane protein transport from the ER to the INM, which allowed investigation of INM targeting and supports a diffusion retention model.
March 2015 - New PublicationHigh-throughput fluorescence correlation spectroscopy enables analysis of proteome dynamics in living cells.
In the link below from the iBioseminar website, watch Jan Ellenberg explaining how to perform high throughput content imaging screening with an update on the recent technologies developed in our lab and EMBL.
Our group is an international interdisciplinary team drawing its members from biology, physics, chemistry, computer science, and engineering. The overarching theme of the lab is to understand the molecular mechanism of the nuclear division cycle in a comprehensive manner in the physiological context of the intact living cell. To achieve this we develop and use a braod range of fluorescence-based imaging technologies to assay the functions of the involved molecular machinery non-invasively, automate imaging to address all its molecular components and computationally process image data to extract biochemical and biophysical parameters in order to generate mechanistic understanding and predictive models. Our biological questions are currently focused on three areas.